Exonuclease I, 20,000 U 1111 65°C for 60sec

65°C for 60sec

Removal of small DNA oligonucleotides (e

Transformation efficiency of Lucigen's electrocompetent bacterial cells for phage display compared to competitor's specification

or for tiling arrays containing overlapping regions of complete chromosomes or genomes

Each NxSeq AmpFREE Low DNA Library Kit contains Enzyme Mix (EM)

Exonuclease I, 20,000 U 1111 65°C for 60secDigest ssDNA, but not dsDNA, in a 3 to 5 direction Specific: Processively digest ssDNA in a 3 to 5 direction without harming dsDNA short 3 overhangs in DNA are not digested Flexible: Active under a wide variety of buffer conditions and can be added directly into most reaction mixes Heat Inactivated: Destroy the enzyme activity by incubating at 80C for 15 minutes Exonuclease I digests ssDNA in a 3 to 5 direction,1 3 but does not digest dsDNA. Although