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In the second study, parameters associated with bone resorption, namely, urinary NTX and deoxypyridinoline and serum TRACP5b were assessed in all subjects as well as bone formation parameters including serum osteocalcin, uncarboxylated osteocalcin (ucOC), total type 1 procollagen-N-propeptide (P1NP), and bone specific alkaline phosphatase (BAP), as were pentosidine, homocystein, intact parathyroid hormone (PTH), 25(OH)VitD, estradiol (E2), and cotinine levels

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tobacco leaves and seeds against the larvae were 0.153 g/mL and 0.188 g/mL, respectively, while the corresponding values for adults were 0.219 g/mL and 0.290 g/mL, indicating comparatively lower toxicity of extracts against adults