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The inhalation time was set at 3 s and two different protocols were applied, according to the selected power level: (1) three puffs of 3 s with 27 s of rest between them for the 15-W and 25-W power levels, (2) two puffs of 3 s with 27 s of rest between them for the 50-W power levels (only two puffs were needed to avoid an overload of the cascade impactor stages)