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injection (5FU) and oral gavage (o.g.) administration (metformin) for 11 days

One aliquot (100 mg) of fecal matter for each experimental sample was resuspended in 8 mL 1 Tris-EDTA pH7,4 supplemented with antiprotease (mini tabletsRoche), transferred into four Precellys tubes (2 mLVK05 supplemented with 100 mL of VK01 glass beads) and disrupted (6700 rpm20 s ON/30 s OFFsix cycles)

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10.1002/glia.10188