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From Days 3 to 8, the medium was switched to Knockout DMEM, 20% Knockout Serum Replacer, beta-mercaptoethanol (BME, 100 M), 1% Glutamax, non-essential amino acids, and 1% DMSO

However, with IBD-current biologic therapies, all these mean some patients primary non-responders, experience a loss of response, intolerance or side effects, defining the urgent unmet need for novel treatments (13, 14)

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Reputable research-grade preparations are commonly described as LPS-free peptides , endotoxin-free peptides , and research peptides endotoxin tested , ensuring suitability for sensitive laboratory assays and minimizing confounding variables in experimental data