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(2016) Glutathione biosynthesis is a metabolic vulnerability in PI(3)K/Akt-driven breast cancer, Nat

How to Inject Peptides Subcutaneously: The 8-Step Protocol The steps below apply to the standard subcutaneous injection technique used for most clinical peptide protocols

References: Pickart, Loren, and Anna Margolina

Consistent with the result that Ser184 phosphorylation was essential for GSTP to enter nucleus, both immunoblotting and confocal microscopy detection demonstrated that GF109203X inhibited LPS-triggered GSTP nucleus translocation, and PMA promoted GSTP to enter the nucleus in LPS-untreated RAW264.7 cells (Figures 6F,G)

For cell number measurements in 6-well plates, the medium was aspirated, the wells were rinsed with PBS and the cells were detached by incubating with 500 l of 0.25% trypsin (Thermo Scientific, 25200056) for 20 min at 37 C