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Therefore, cancer cells express hypoxia-inducible factor 1 (HIF-1) and secrete VEGF to promote angiogenesis (24)

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No universally standardized dosing protocol exists yet, so working with a qualified provider to personalize frequency and dosage remains essential

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After treatment, cells were immediately analyzed for apoptosis and the cell cycle using a BD FACSCelesta Cell Analyzer with an Annexin V-FITC/PI Apoptosis Detection Kit and PI/RNase Staining Buffer (BD Biosciences, Franklin Lakes, NJ, USA), respectively, according to the manufacturers instructions