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2 S-producing enzyme SELENBP1 and decreased expression of CBS, forms a reciprocal regulatory network that mirrors the H 2 S detoxification mechanism of normal colonic crypt epithelium

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The transfection reagent Lipofectamine 3000 (Invitrogen, Waltham, MA) was similarly diluted in Opti-MEM at a transfection concentration of 50 nM