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Within the GPx family, which contains selenocysteine in the active site, it has been proposed that the protective functions of GPx2 in suppressing high oxidative stress may be more important than those of other GPxs

valine, V), and four polar amino acids (glutamine, Q

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Briefly, cells pretreated with 4 mM glutamine (Gln) and 0 mM Gln, respectively, were cross-linked with formaldehyde at 37 C for 10 min, followed by the addition of glycine solution to quench the cross-linking reaction