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This metabolic dependency of transformed cells on glutamine constitutes the recently defined glutamine addiction 8

Briefly, total RNA was extracted from synovial tissues and FLS using TRIzol

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Although we did not assess the differentiation potential to other lineages such as osteogenesis and adipogenesis according to cellular GSH levels, we found that high GSH levels increased the chondrogenic potential of not only hES-MSCs but also of SDSCs, UC-MSCs, and ADSCs, highlighting the ability of FreSHtracer to selectively isolate highly functional stem cells regardless of the tissue source

Simons-Weidenmaier NS, Weber M, Plappert CF, Pilz PK, Schmid S