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To test for functionality of the membrane-bound catalytic domains, mesophyll protoplasts were prepared from g6pd5-1 g6pd6-2 double-mutant plants lacking cytosolic G6PDH activity (similar to the g6pd5-1 g6pd6-1 combination used by Wakao et al., Figure 5B)

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doi: 10.1210/jc.2008-2235 145 SinghSModiSBaggaDKaurPShankarLRKhushuS

A-GSH targeting resulted in a time-dependent increase in the cellular internalization of 3-PLGs conjugated with active agents (Figs