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The corresponding running buffer was composed of 50 mM sodium phosphate, pH 6.5, 150 mM NaCl and 1 mM DTT

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Agomelatine (activity at the serotonin-2C receptor) functions as an anti-depressant, ramelteon (selective MT1/MT2 agonist) is marketed for use in sleep onset insomnia, tasimelteon (MT1/MT2 agonist), is commercialized for the treatment of circadian rhythm disorders particularly non-24h sleep wake disorder (113, 114)