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MPP + treatment significantly reduces TIGAR expression in HT22 cells, downregulates NADPH, GPX4, and GSH levels, upregulates GSSG levels and the GSSG/GSH ratio, decreases adenosine triphosphate (ATP) production, and promotes neuronal ferroptosis, iron-dependent lipid peroxidation, and mitochondrial dysfunction, thereby exacerbating neuronal cytotoxicity

For experimental details see Materials and Methods

This documentation helps troubleshoot effectiveness issues and refine storage practices over time

The method involves the formation of a colored complex of LPO products with thiobarbituric acid (TBA) in an acidic environment

This mechanism is supported by peer-reviewed research, including studies by Khavinson and colleagues published in gerontology journals, confirming its biological effects