An inverted fluorescence microscope (Nikon, DS-Ri2, Japan) was used to obtain fluorescence images
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DOX dissolved effectively in all selected solvent systems except PEG400
1 Introduction The unprecedented pace of industrialization and urbanization over recent decades has introduced a vast array of chemical contaminants into our environment, among which environmental endocrine disruptors (EEDs) have emerged as a particularly concerning threat to human reproductive health ( 1.1 Sources and classification of EEDs Environmental endocrine disruptors encompass a remarkably diverse array of chemical compounds that can be systematically categorized into three distinct groups based on their origin, chemical properties, and environmental behavior: Heavy Metals and Metalloids represent one of the most persistent categories of reproductive toxicants
In laboratory settings, it is the standard vehicle for reconstituting lyophilized peptides and proteins, ensuring that the chemical stability and pH environment remain optimal for in-vitro analysis without introducing bacterial contaminants that could degrade sensitive compounds
