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When malabsorption is the issue, simply eating more B12-rich foods or taking standard oral supplements might not be enough

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To study gene expression, total RNA was extracted from half-colon samples using phenol-chloroform methods and purified with lithium chloride precipitation, which is particularly important to remove residual DSS polysaccharides that can interfere with downstream reactions

Urgent care may decline prednisone if you've had recent courses (risk of adrenal suppression), have uncontrolled diabetes, active infections that steroids could worsen, or conditions requiring specialist management