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The primary measured outcomes included the neural functional deficit score, infarct volume, brain water content, cell viability, apoptotic cells, terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end labeling (TUNEL)-positive cells, B-cell lymphoma-2 (Bcl-2) level, and tumor necrosis factor- (TNF-) level

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This comprehensive matrix-enhancing profile distinguishes GHK-Cu from selective collagen-stimulating agents, offering broader regenerative effects across multiple matrix components