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Bound FcRn was detected either by a horseradish peroxidase (HRP)-conjugated anti-GST antibody from goat (Rockland Immunochemicals, 200-301-200) (diluted 1:5000 in PBS/T/S) or streptavidin-AP (diluted 1:5000 in PBS/T/S) and visualized by addition of TMB substrate (CalBiochem) or p-nitrophenylphosphate (AP substrate) (Sigma-Aldrich) diluted to 10 g/mL in diethanolamine buffer

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Body composition: Tesamorelin (FDA-approved GHRH analog) shows significant reductions in visceral adipose tissue (Falutz et al., 2007), supporting that GHRH-axis stimulation improves body composition

Studies show that while GHRP-2 and GHRP-6 administration resulted in increased plasma levels of ACTH and cortisol, ipamorelin did not release ACTH or cortisol at levels significantly different from those observed following growth hormone-releasing hormone (GHRH) stimulation

rats) used [64]