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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

Directions for Use Prime pen before first use Standard dose per injection: 250500 mcg (0.250.5 mg) (most common effective range for tissue repair studies) 250 mcg (0.25 mg): 6 clicks (0.25 0.0417 6) 500 mcg (0.5 mg): 12 clicks (0.5 0.0417 12) Frequency: 12 times daily (e.g., morning and evening

FDA material governs the United States rather than the United Kingdom, but it provides a useful illustration of the unresolved safety and quality questions surrounding the compound

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