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For iHLO differentiation, iPSCs were dissociated with Versene (Thermo Fisher Scientific, catalog: 15040066) on Day 0 and plated into an AggreWell plate (STEMCELL Technologies, catalog: #34421) in Stemflex Medium (Thermo Fisher Scientific) to form aggregates, which were then transferred to an ultralow attachment dish on Day 1 and cultured in RPMI1640 supplemented with B27 supplement minus insulin (RPMI/B27-Insulin), CHIR99021 (1 M), Activin A (100 ng/mL), WNT3a (50 ng/mL), and Rock Inhibitor (Y27632, 5 M)

The right temporal muscle was dissected, the temporal bone dried, and a laser Doppler flow (LDF) probe was attached to the temporal bone to monitor regional cerebral blood flow (rCBF) during the procedure

Research Use Only GLOW and each of its three components are sold labeled for laboratory research use only

2022 Dec 12;10(12):3221

Atrophic gastritis is more common in elderly people of African-American or Northern-European descent