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2017 Dec 12 2 Katz JN, Zimmerman ZE, Mass H, Makhni MC

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Freezing causes ice crystal formation that physically disrupts peptide chains

A consistent model goes with ligation leading to vessel injury, exposure of tissue factors, stasis, thrombosis, hemodynamic changes, arterial hypotension, and in particular, venous hypertension, which approximates values over 20 mm Hg in the inferior caval vein, thereby being roughly four times higher than those in normal rats