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These minerals penetrate the skin, helping to repair damaged cells, reduce inflammation, and restore skin vitality

Research interest nonetheless expanded considerably through the 1980s and 1990s, as investigators began exploring DSIP's broader neuroendocrine roles, including its interactions with the HPA axis, circadian timing systems, and stress-response pathways, establishing the compound as a multifunctional regulatory neuropeptide whose biological significance extends well beyond its original sleep-focused characterization

Oligo dT primers and primers for real-time PCR were obtained from Integrated DNA Technologies (IDT, Coralville, IA, USA)