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Transfer the solution slowly along the vial wall to support controlled mixing and minimize unnecessary agitation of the lyophilized material

Total RNA isolated from the cell samples was processed using GeneChip 3' IVT PLUS Reagent Kit (Affymetrix

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This aligns with research showing that vegetarians and vegans have lower levels of EPA & DHA compared to those who eat fish 4 Blood docosahexaenoic acid and eicosapentaenoic acid in vegans: Associations with age and gender and effects of an algal-derived omega-3 fatty acid supplement Sarter et al