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The mobile phases consisted of solvent A (0.1% formic acid in water) and solvent B (0.1% formic acid in methanol)

Membranes were stripped for 20 min at room temperature using a Blot Restore Membrane Rejuvenation kit (Sigma-Aldrich, 2520-M), then washed twice with TBS-T, re-blocked with BSA 5% for 30 min and incubated with mouse-anti--actin (Sigma-Aldrich, A1978) 1:3,000 in BSA 2.5% for 4 h at room temperature and then incubated with a sheep-anti-mouse (Amersham, NA931) secondary antibody at 1:5,000 in BSA 2.5% for 2 h

The truth is, the exact vitamin B12 injection dosage for adults and children matters less than the frequency of the shots

Keep it sealed and dry Powder pulls moisture from the air, which speeds degradation