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Helwig NE, Hong S, Hsiao-wecksler ET

Drops (2 L) consisting of a 1:1 ratio of C20A mutant OsDHAR protein (21 mg/mL in 20 mM Tris-HCl, pH 8.0 and 150 mM NaCl) and crystallization solution (100 mM Bis-Tris HCl, pH 6.16.8, 28% (w/v) PEG 3350 and 10 mM CaCl 2 ) were equilibrated against 0.5 mL of the same solution

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As a result, this alteration stops Nrf2 from being ubiquitinated, which allows it to go into the nucleus and attach to antioxidant response elements (ARE) found in the promoter regions of antioxidant genes to increase their production