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Chitosan nanoparticles with positive charge can bind to fungal cell walls and cell membranes with negative charge, which will lead to increased permeability of cell envelopes and leakage of cytoplasmic contents from the cells, including ions and protein [66]

Meanwhile, the flow cytometry results revealed a dramatic increase in the levels of C11-BODIPY (a maker of ferroptosis), ROS and Fe 2+ in FAC-treated primary hepatocytes (Fig

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These free radicals are unstable molecules missing an electron, and if left unchecked, they will aggressively steal electrons from nearby cellular structures, causing severe damage to delicate lipid membranes, vital proteins, and even cellular DNA

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