The tissue and cell disruption during processing (e.g., mixing, cutting, crushing, freezing/thawing, and high pressure treatment) makes native polyglutamyl folates accessible for endogenous conjugases (-glutamyl hydrolases) and results in the hydrolysis to monoglutamyl folates in vegetables (e.g., leeks, cauliflower, broccoli, spinach, soybeans, green beans, cowpea leaves, turnip, and carrot) and fruits (e.g., orange, papaya, sweet cherry, strawberry, and blackberry)
It is not approved for therapeutic use and remains for investigational and laboratory applications
The beads were washed with wash buffer (20 mM HEPES-NaOH pH 7.4, 200 mM NaCl, 2 mM MgCl 2 , 0.5 mM TCEP) supplemented with 2 mM ATP
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This provides sufficient nutrients without excessive calorie intake