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Szilagyi et al

Regardless of the method, removal or reduction of GSSG will affect the [GSH]:[GSSG] ratio

At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS

18 For example, in one study, researchers discovered that when GSH is depleted in the hippocampus regions of an elderly person the healthy brain suffers mild cognitive impairment, which is known to be present in the earlier stages of AD

An alternative interpretation for the first phase could be a rapid noncovalent interaction between PfGrx and GSSG in the vicinity of roGFP2, however, this seems less likely considering the smaller values for the structurally similar PfGrx mutants or, in particular, the rxYFP fusion constructs with ScGrx1 (Table 1)