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Then, NAPQI-modified proteins were digested via two proteases (trypsin and pepsin) in parallel and analyzed by two-dimensional LC-MS/MS, using high-pH reversed-phase (RP) offline fractionation

Conversely, empty capsids produced from the cell culture infected at 34C (empty capsid B, Scheme 1) co-eluted with infectious CVA21 virions and required either further optimization of GSH chromatography parameters or development of a polishing chromatography step to clear residual empty capsids

A Completely in Vitro Ultrahigh-Throughput Droplet-Based Microfluidic Screening System for Protein Engineering and Directed Evolution

2022 Oct 8;11(19):3156

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