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The PDE5 inhibitory constituents of medicinal plant extracts include alkaloids, phenolics, and polycyclic aromatic hydrocarbons

HpGroEL and HpTpx are covalently bound and inhibited by Metro and Metro-P3 after reductive activation As binding of Metro and derivatives to HpGroEL and HpTpx requires the in situ activation of the nitro group under microaerophilic or anaerobic conditions, direct inhibition and binding assays with purified enzymes in vitro were challenging (Supplementary Figs