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A wide range of prior efforts have focused on creative ways to measure the accumulation of small molecules, including coupling of periplasmic accumulation to b-lactamase activity 79 , split luciferase 14 , modification of antibiotics with fluorescent handles 80 , LC-MS-based quantification 15,41,42,81,82 , electrophysiology using planar lipid bilayers 83,84 , and microfluorospectrometry 85,86,87

This step ensures that the supplement will not interact negatively with any existing medications or underlying medical conditions

bioRxiv , 025866

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GPX3 mRNA is present in renal proximal tubular epithelial cells, and GPX3 may have specific antioxidant functions in the renal tubules or extracellular spaces due to the higher concentration of GSH in the kidneys